anti cd21 bl13 fluidigm Search Results


anti human cd21 bl 13 152sm  (fluidigm)
93
fluidigm anti human cd21 bl 13 152sm
DDR in naïve lymphocyte subsets is differential to mature memory populations. PBMCs obtained from 26 healthy donors were irradiated with 2Gy and fixed at indicated time points. Surface markers of lymphocyte subsets and intranuclear DDR biomarkers were assessed by mass cytometry. Fold inductions of γH2AX, p-ATM, p-CHK2, and p53 were calculated in CD45 + lymphocyte subsets, based on mean fluorescence intensities normalized on unirradiated samples. T cell subsets were characterized as CD3 + , CD3 + CD4 + , CD3 + CD8 + , CD45RA + CCR7 + (naïve CD4/CD8), CD45RO + CCR7 +/- (central and effector memory CD4/CD8) (A) . NK lymphocyte subsets were defined as CD3 - CD56 bright CD16 - , CD3 - CD56 bright CD16 + , CD3 - CD56 dim CD16 + (B) , which were further stratified to CD57 expression on CD56 bright CD16 + and CD56 dim CD16 + subsets. CD3 - B lymphocytes were characterized as CD19 + CD20 + , CD27 - IgD + (naïve B), CD27 + (memory B), CD27 + IgM + (unswitched memory B) CD27 + IgM - IgGκ + (class switched memory B IgGκ), CD27 + IgM - IgGΛ + (class switched memory B IgGΛ), CD27 + IgM + IgD + (Marginal Zone (MZ)-like B), IgM ++ CD38 ++ (transitional B), CD27 + IgM + IgD - (IgM only B), CD27 - IgM - IgD - (atypical memory B), CD19 + CD20 - CD27 + CD38 + IgM + (unswitched plasmablasts), CD19 + CD20 - CD27 + CD38 + IgM - (class switched plasmablasts), and <t>CD21</t> low CD38 low B cells. (C) Bars represent mean values of fold induction; error bars represent standard deviations. Significance is shown for naïve T and B cell subsets compared to memory subsets, and immature CD56 bright CD16 - to mature CD56 bright CD16 + and CD56 dim CD16 + NK lymphocytes (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001).
Anti Human Cd21 Bl 13 152sm, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd21+bl13+fluidigm/Anti-Human+CD21+(BL13)-152Sm/pmc08478158-58-104-108
Average 93 stars, based on 1 article reviews
anti human cd21 bl 13 152sm - by Bioz Stars, 2026-09
93/100 stars
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DDR in naïve lymphocyte subsets is differential to mature memory populations. PBMCs obtained from 26 healthy donors were irradiated with 2Gy and fixed at indicated time points. Surface markers of lymphocyte subsets and intranuclear DDR biomarkers were assessed by mass cytometry. Fold inductions of γH2AX, p-ATM, p-CHK2, and p53 were calculated in CD45 + lymphocyte subsets, based on mean fluorescence intensities normalized on unirradiated samples. T cell subsets were characterized as CD3 + , CD3 + CD4 + , CD3 + CD8 + , CD45RA + CCR7 + (naïve CD4/CD8), CD45RO + CCR7 +/- (central and effector memory CD4/CD8) (A) . NK lymphocyte subsets were defined as CD3 - CD56 bright CD16 - , CD3 - CD56 bright CD16 + , CD3 - CD56 dim CD16 + (B) , which were further stratified to CD57 expression on CD56 bright CD16 + and CD56 dim CD16 + subsets. CD3 - B lymphocytes were characterized as CD19 + CD20 + , CD27 - IgD + (naïve B), CD27 + (memory B), CD27 + IgM + (unswitched memory B) CD27 + IgM - IgGκ + (class switched memory B IgGκ), CD27 + IgM - IgGΛ + (class switched memory B IgGΛ), CD27 + IgM + IgD + (Marginal Zone (MZ)-like B), IgM ++ CD38 ++ (transitional B), CD27 + IgM + IgD - (IgM only B), CD27 - IgM - IgD - (atypical memory B), CD19 + CD20 - CD27 + CD38 + IgM + (unswitched plasmablasts), CD19 + CD20 - CD27 + CD38 + IgM - (class switched plasmablasts), and CD21 low CD38 low B cells. (C) Bars represent mean values of fold induction; error bars represent standard deviations. Significance is shown for naïve T and B cell subsets compared to memory subsets, and immature CD56 bright CD16 - to mature CD56 bright CD16 + and CD56 dim CD16 + NK lymphocytes (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001).

Journal: Frontiers in Immunology

Article Title: Differential DNA Damage Response of Peripheral Blood Lymphocyte Populations

doi: 10.3389/fimmu.2021.739675

Figure Lengend Snippet: DDR in naïve lymphocyte subsets is differential to mature memory populations. PBMCs obtained from 26 healthy donors were irradiated with 2Gy and fixed at indicated time points. Surface markers of lymphocyte subsets and intranuclear DDR biomarkers were assessed by mass cytometry. Fold inductions of γH2AX, p-ATM, p-CHK2, and p53 were calculated in CD45 + lymphocyte subsets, based on mean fluorescence intensities normalized on unirradiated samples. T cell subsets were characterized as CD3 + , CD3 + CD4 + , CD3 + CD8 + , CD45RA + CCR7 + (naïve CD4/CD8), CD45RO + CCR7 +/- (central and effector memory CD4/CD8) (A) . NK lymphocyte subsets were defined as CD3 - CD56 bright CD16 - , CD3 - CD56 bright CD16 + , CD3 - CD56 dim CD16 + (B) , which were further stratified to CD57 expression on CD56 bright CD16 + and CD56 dim CD16 + subsets. CD3 - B lymphocytes were characterized as CD19 + CD20 + , CD27 - IgD + (naïve B), CD27 + (memory B), CD27 + IgM + (unswitched memory B) CD27 + IgM - IgGκ + (class switched memory B IgGκ), CD27 + IgM - IgGΛ + (class switched memory B IgGΛ), CD27 + IgM + IgD + (Marginal Zone (MZ)-like B), IgM ++ CD38 ++ (transitional B), CD27 + IgM + IgD - (IgM only B), CD27 - IgM - IgD - (atypical memory B), CD19 + CD20 - CD27 + CD38 + IgM + (unswitched plasmablasts), CD19 + CD20 - CD27 + CD38 + IgM - (class switched plasmablasts), and CD21 low CD38 low B cells. (C) Bars represent mean values of fold induction; error bars represent standard deviations. Significance is shown for naïve T and B cell subsets compared to memory subsets, and immature CD56 bright CD16 - to mature CD56 bright CD16 + and CD56 dim CD16 + NK lymphocytes (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001).

Article Snippet: The following surface antibodies were used at concentrations of 1μl/100μl staining buffer: Mouse anti-human CD45 (HI30)-154Sm (Fluidigm), anti-human CD3 (UCHT1)-170Er (Fluidigm), anti-human CD4 (SK3)-174Yb (Fluidigm), anti-Human CD8 (Sk1)-168Er (Fluidigm), anti-human CD45RA (HI100) 169Tm (Fluidigm), anti-human CD45RO (UCHL1) 164-Dy (Fluidigm), anti-human CD197/CCR7 (G053H7) 159Tb (Fluidigm), anti-human CD69 (FN50) 144Nd (Fluidigm), anti-human CD56 (HCD56) 176Yb (Fluidigm), anti-Human CD16 (3G8)-165Ho/Fluidigm, anti-human CD57 (HCD57) 163Dy (Fluidigm), anti-human CD19 (HIB19) 142Nd (Fluidigm), anti-human CD20 (2H7) 171Yb (Fluidigm), anti-human IgD (IA6-2) 146Nd (Fluidigm), anti-human IgM (MHM-88) 172Yb (Fluidigm), anti-human IgG kappa (MHK-49) 160Gd (Fluidigm), anti-human IgG lambda (MHL-38) 151Eu (Fluidigm), anti-human CD27 (L128) 158Gd (Fluidigm), anti-human CD38 (HIT2) 167Er (Fluidigm), anti-human CD21 (BL-13) 152Sm (Fluidigm).

Techniques: Irradiation, Mass Cytometry, Fluorescence, Expressing